The new fast resonant scanning Leica SP8 confocal microscope is a high-tech powerhouse. The resonant scanning unit allows confocal images at 28 frames per second full-frame, and faster for smaller images. It is equipped with a cryo-incubator stage allowing stable temperature control from -2°C to 50°C enabling live real-time long time-lapse imaging. The recent upgrade has added FLIM (fluorescence lifetime imaging microscopy) capabilities!
This amazing new microscope uses Stimulated Emission Depletion to capture super resolution images with as fine of resolution as 30-50 nm, which is a huge improvement compared to the typical resolution limitation of 200-300 nm with a normal confocal microscope. It is compatible with fixed and live samples and sample prep is similar to confocal. Certain fluorophores work better for STED than others, so be sure to consult with the facility director for help planning your sample preparation. Also works great as a normal confocal microscope, especially for low signal imaging!
The Nanolive 3D Cell Explorer is a novel new instrument that uses holographic tomography to generate a three-dimensional image of the sample with contrast determined by refractive index. It works primarily with adherent cell cultures where the instrument makes many subcellular structures immediately visible label-free. A 30 micrometer stack of images can be collected every 2 seconds. It can keep cells alive in a healthy state for days at a time allowing incredibly long timelapses.
This lightsheet microscope is fully equipped and can be used for fast imaging of samples with a lot of 3-dimensional depth. Most commonly used for imaging embryo development, and other multicellular organsims live or fixed. Excellent environmental chamber controls for temperature, immersion media, and CO2. Instead of imaging on a slide, the sample is embedded in a column of agarose.
This Olympus inverted compound microscope is equipped with high quality objectives, fluorescence and a Qimaging scientific CMOS camera allowing high frame rates even in low light conditions. The filters, objectives, and z-position are all motorized for automated image collection.
These 2 computer workstation runs current Imaris software with the Imaris Measurement Pro, Filament Tracer, Cell, Colocalization, Track, Vanage and XT modules. They both also have Fiji (ImageJ). Hercules 2 has Zeiss Zen, Leica LASX, and Huygens deconvolution software.