Identification and expression of small multidrug resistance (SMR) transporters in early-branching anaerobic fungi

TitleIdentification and expression of small multidrug resistance (SMR) transporters in early-branching anaerobic fungi
Publication TypeJournal Article
Year of Publication2023
AuthorsSeppälä S, Gierke T, Schauer EE, Brown JL, O'Malley MA
JournalProtein Science
Volumen/a
Paginatione4730
KeywordsAnaerobic fungi, bioprocessing, Membrane proteins, small multidrug resistance transporters, Yeast
Abstract

Abstract Membrane-embedded transporters impart essential functions to cells as they mediate sensing and the uptake and extrusion of nutrients, waste products, and effector molecules. Promiscuous multidrug exporters are implicated in resistance to drugs and antibiotics and are highly relevant for microbial engineers who seek to enhance the tolerance of cell factory strains to hydrophobic bioproducts. Here, we report on the identification of Small Multidrug Resistance (SMR) transporters in early-branching anaerobic fungi (Neocallimastigomycetes). The SMR class of transporters is commonly found in bacteria but has not previously been reported in eukaryotes. In this study, we show that SMR transporters from anaerobic fungi can be produced heterologously in the model yeast Saccharomyces cerevisiae, demonstrating the potential of these proteins as targets for further characterization. The discovery of these novel anaerobic fungal SMR transporters offer a promising path forward to enhance bioproduction from engineered microbial strains. This article is protected by copyright. All rights reserved.

URLhttps://onlinelibrary.wiley.com/doi/abs/10.1002/pro.4730
DOI10.1002/pro.4730