|Title||A Scribble/Cdep/Rac pathway controls follower-cell crawling and cluster cohesion during collective border-cell migration|
|Publication Type||Journal Article|
|Year of Publication||2022|
|Authors||Campanale JP, Mondo JA, Montell DJ|
|Keywords||apicobasal polarity, border cells, Cdep, collective cell migration, FARP2, par complex, Rac1, RhoGTPases, Scribble complex|
Summary Collective cell movements drive normal development and metastasis. Drosophila border cells move as a cluster of 6–10 cells, where the role of the Rac GTPase in migration was first established. In border cells, as in most migratory cells, Rac stimulates leading-edge protrusion. Upstream Rac regulators in leaders have been identified; however, the regulation and function of Rac in follower border cells is unknown. Here, we show that all border cells require Rac, which promotes follower-cell motility and is important for cluster compactness and movement. We identify a Rac guanine nucleotide exchange factor, Cdep, which also regulates follower-cell movement and cluster cohesion. Scribble, Discs large, and Lethal giant larvae localize Cdep basolaterally and share phenotypes with Cdep. Relocalization of Cdep::GFP partially rescues Scribble knockdown, suggesting that Cdep is a major downstream effector of basolateral proteins. Thus, a Scrib/Cdep/Rac pathway promotes cell crawling and coordinated, collective migration in vivo.