Rapid analysis of cell-generated forces within a multicellular aggregate using microsphere-based traction force microscopy

TitleRapid analysis of cell-generated forces within a multicellular aggregate using microsphere-based traction force microscopy
Publication TypeJournal Article
Year of Publication2020
AuthorsKaytanli B, Khankhel AH, Cohen N, Valentine MT
JournalSoft Matter
Volume16
Pagination4192-4199
Abstract

We present a new approach to measuring cell-generated forces from the deformations of elastic microspheres embedded within multicellular aggregates. By directly fitting the measured sensor deformation to an analytical model based on experimental observations and invoking linear elasticity, we dramatically reduce the computational complexity of the problem, and directly obtain the full 3D mapping of surface stresses. Our approach imparts extraordinary computational efficiency, allowing tractions to be estimated within minutes and enabling rapid analysis of microsphere-based traction force microscopy data.

URLhttp://dx.doi.org/10.1039/C9SM02377A
DOI10.1039/C9SM02377A