The Thiol-Rich Interlayer in the Shell/Core Architecture of Mussel Byssal Threads.

TitleThe Thiol-Rich Interlayer in the Shell/Core Architecture of Mussel Byssal Threads.
Publication TypeJournal Article
Year of Publication2019
AuthorsValois E, Hoffman C, DeMartini DG, J Waite H
JournalLangmuir
Date Published2019 Aug 23
ISSN1520-5827
Abstract

The mussel byssus thread is an extremely tough core-shelled fiber that dissipates substantial amounts of energy during tensile loading. The mechanical performance of the shell is critically reliant on 3,4-dihydroxyphenylalanine's (Dopa) ability to form reversible iron-catecholate complexes at pH 8. However, the formation of these coordinate cross-links is undercut by Dopa's oxidation to Dopa-quinone, a spontaneous process at seawater conditions. The large mechanical mismatch between the cuticle and the core lends itself to further complications. Despite these challenges, the mussel byssus thread performs its tethering function over long periods of time. Here, we address these two major questions: (1) how does the mussel slow/prevent oxidation in the cuticle, and (2) how is the mechanical mismatch at the core/shell interface mitigated? By combining a number of microscopy and spectroscopy techniques we have discerned a previously undescribed layer. Our results indicate this interlayer is thiol rich and thus will be called the thiol-rich interlayer (TRL). We propose the TRL serves as a long-lasting redox reservoir as well as a mechanical barrier.

DOI10.1021/acs.langmuir.9b01844
Alternate JournalLangmuir
PubMed ID31405280