Title | Enhanced Neuronal Regeneration in the CAST/Ei Mouse Strain Is Linked to Expression of Differentiation Markers after Injury |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Lisi V, Singh B, Giroux M, Guzman E, Painter MW, Cheng Y-C, Huebner E, Coppola G, Costigan M, Woolf CJ, Kosik KS |
Journal | Cell Reports |
Volume | 20 |
Pagination | 1136 - 1147 |
ISSN | 2211-1247 |
Keywords | miR-7048 |
Abstract | Summary Peripheral nerve regeneration after injury requires a broad program of transcriptional changes. We investigated the basis for the enhanced nerve regenerative capacity of the CAST/Ei mouse strain relative to C57BL/6 mice. \{RNA\} sequencing of dorsal root ganglia (DRG) showed a CAST/Ei-specific upregulation of Ascl1 after injury. Ascl1 overexpression in \{DRG\} neurons of C57BL/6 mice enhanced their neurite outgrowth. Ascl1 is regulated by miR-7048-3p, which is downregulated in CAST/Ei mice. Inhibition of miR-7048-3p enhances neurite outgrowth. Following injury, CAST/Ei neurons largely retained their mature neuronal profile as determined by single-cell RNA- seq, whereas the C57BL/6 neurons acquired an immature profile. These findings suggest that one facet of the enhanced regenerative phenotype is preservation of neuronal identity in response to injury. |
URL | http://www.sciencedirect.com/science/article/pii/S2211124717309622 |
DOI | 10.1016/j.celrep.2017.07.010 |